Defend Effects of Melatonin on Protoplasts of Gentiana macrophylla Under UV-B Irridiation
Bibliographic record
Abstract
Comet assay,named single cell gel electrophoresis assay( SCGE),was used to analyze the effect of melatonin( MEL) on the DNA repair ability of Gentiana macrophylla Pall. protoplasts. Cell was irradiated with different doses of ultraviolet-B radiation,DNA damage is indicated by Tail DNA%. MEL was added to the protoplast suspension at 0,1,10 μmol·L- 1final concentration,the irradiance of UV-B is 0. 5 W·m- 2. Experiment was divided into two parts. The first group,protoplasts was radiated by UV-B with different times( 0,5,10,30,60,120 s) and incubated in the dark60 min,electrophoresis. The second group,protoplast was irradiated by UV-B 40 s and incubated in the dark with different times( 0,1,2,3,4,6h),and then electrophoresis. In the range of 5 to 30 s,the same radiation dose,the higher concentration of MEL,the lower Tail DNA%. After cells were cultured for 1 to 6 hours,Tail DNA% which the samples was treated with MEL were always lower than the positive control's,and the Tail DNA% showed an inverse relationship with MEL concentration. A dose-related protective effect of MEL was observed in our experiment and MEL reduces damage of cells after irradiation by UV-B,promote the repair of DNA. In the study,the first use of SCGE technology further revealed that MEL improved the resistance of plant to environmental stress.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".