Cloning and sequence analysis of Cyt b gene in four Sparidae fishes in Zhoushan sea area
Bibliographic record
Abstract
The complete sequences of mitochondrial Cyt b gene in Pagrus pagrus,Sparus macrocephalus,Acanthopagrus latus and Parargyrops edita in Zhoushan sea area were amplified,cloned and sequenced.The 1 141 bp Cyt b gene sequences of these four species were obtained.Compared with the same DNA fragment recorded in the GeneBank,the sequences of 1 141 bp DNA had 292 nucleotide variation sites,among which 186 parsimony informative sites were observed.Transitions were discovered to be more than transversions,and most of the base substitutions occurred at the third locus of the codon.The genetic distance among 4 Sparidae species ranged from 0.096 7 to 0.227 5.The shortest distance occurred between P.pagrus and P.edita,being 0.096 7,while the longest one between S.macrocephalus and A.latus,being 0.227 5.Moreover,the transition-transversion ratio of the sequence differentiation of 4 species ranged from 2.293 0 to 7.358 7.The NJ phylogenetic tree revealed that 25 species were divided into two clades,and obvious genetic divergence existed between fishes of Sparidae and the other fishes of Percoidea.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".