Enzymatic Analysis of cDNA-Expressed Human CYPIAI, CYPlA2, and CYPIBI with 7-Ethoxyresorufin as Substrate
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
The human CYP1 family consists of at least three proteins, CYP1A1, CYP1A2, and CYP1B1 (1), CYP1A1 is absent or present at very low levels in human liver (2, 3), but its expression is readily detectable in lung (4, 5). By contrast, CYP1A2 is constitutively expressed in human liver (2, 3) and is absent in lung (4, 5). CYP1B1 is primarily an extrahepatic P450, as suggested by the findmg that CYP1B1 mRNA is present in much greater abundance in tissues such as kidney, prostate, and breast than in liver (6). Exposure to polycyclic aromatic hydrocarbons such as those found in cigarette smoke induces the expression of both CYP1A1 and CYP1A2 (3, 7). CYP1B1 is also inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), as demonstrated by cell-culture experiments (8, 9). The chemical inhibitor a-naphthoflavone, which is a potent inhibitor of CYP1A1 and CYP1A2 (10), also inhibits CYP1B1 and with semilar efficacy and potency (11). cDNA-expressed CYP1A1, CYP1A2 and CYP1B1 are each active in the oxidation of theophylline (11), caffeine (11, 12), estradiol (13, 14), benzo[a]pyrene (11) and 7-ethoxyresorufin (11). With 7-ethoxyresorufin as substrate, the rank order of specific activity (pmol/min/nmol P450) is CYP1A1 > CYP1B1 > CYP1A2 (11). 7-Ethoxyresorufin O-deethylation activity can be a useful and convenient catalytic monitor when conducting a comparative study using a panel of these three recombinant CYP1 enzymes (see Note 1). Immunoinhibition experiments with inhibitory CYP1A-selective antibodies have suggested that CYP1A2 is a major contributor to 7-ethoxyresorufin O-deethylase activity in human liver microsomes (15, 16).
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it