Assays of Lecithin Cholesterol Acyltransferase (LCAT)
Bibliographic record
Abstract
Lecithin cholesterol acyltransferase (LCAT, EC 2.3.1.43), a plasma enzyme secreted by hepatocytes, catalyzes the transfer of acyl group of fatty acids from the 2-sn position of lecithin to the 3-hydroxy group of cholesterol. Ordinarily the reaction takes place mostly on the surface of high-density lipoproteins (HDL). In human plasma, this reaction is the major source of cholesteryl esters (CE) and has a crucial role in the remodeling of plasma lipoproteins and in reverse cholesterol transport A number of reviews describe the properties, mechanism of action, and methods of LCAT determination (, , , , ). Whereas there is a definite potential for the clinical use of LCAT assays, its use is not widespread, being now limited to the diagnosis of LCAT deficiency syndromes, such as LCAT deficiency and Fish Eye Disease. After almost 30 yr of efforts to reach consensus on the best assessment of LCAT activity and/or the rate of cholesterol esterification in plasma, there is still considerable ambiguity and misunderstanding surrounding the assays used today. It is of particular importance to find a method that reflects the physiological process and indicates the amount of produced CE. The LCAT reaction in plasma consisis of three phases. Each of them can be influenced by reaction conditions during in vitro assay and thus affect the final result. The stages of the reaction are as follows:
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.003 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".