P07.02 Evaluation of the hologic transcription mediated amplification assay for detection of<i>mycoplasma genitalium</i>from urine samples
Bibliographic record
Abstract
Introduction M. genitalium is an emerging sexually transmitted pathogen, with a strong association with urethritis, cervicitis and pelvic inflammatory disease. Detection of this bacterium by routine culture is not practical and routine diagnosis and screening for M. genitalium by molecular techniques has been hampered by lack of readily available commercial assays. A preliminary version of a commercial amplification assay is currently available on the Panther platform and was evaluated against an in-house qPCR assay currently in use for routine diagnostics. Methods Overall 1000 consecutive urine samples from men and women were utilised for this evaluation. Over the course of 3 months, urine samples were obtained from consecutive symptomatic men and women being screened for M. genitalium at Melbourne Sexual Health Centre, as well as women being screened prior to termination of pregnancy at the Royal Women’s Hospital, Melbourne. The primary Hologic assay targeting 80bp region of 16s rRNA was compared to the in-house diagnostic assay which targets a 517bp region of 16S gene, as well as a second 16s rRNA target available on the Hologic platform. Results The comparison of the two targets available on the Hologic platform showed very high correlation (k = 0.97 95% CI 0.93–1.00). Comparison of primary Hologic assay to in-house 16S qPCR assay, also showed very good correlation (K = 0.84 95% CI 0.75–0.93). Overall, both primary and secondary Hologic assays on Panther were more sensitive than the 16S qPCR for detection of M. genitalium in urine specimens. Conclusion The M. genitalium assay on the Hologic platform integrated well with the laboratory procedures allowing rapid testing and possibility of rapid and accurate reporting using integration with laboratory information system. Overall the Hologic assay for detection of M. genitalium offers a simple, accurate and sensitive platform for diagnostic laboratories for detection of this important upcoming pathogen. Disclosure of interest statement Hologic supplied the diagnostic kits and the Panther platform to conduct this study.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.009 | 0.009 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.002 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".