MétaCan
Menu
Back to cohort
Record W2411433299 · doi:10.1007/978-1-59745-553-4_1

PCR–ELISA for High-Throughput Blood Group Genotyping

2009· review· en· W2411433299 on OpenAlexaff
Maryse St‐Louis

Bibliographic record

VenueMethods in molecular biology · 2009
Typereview
Languageen
FieldMedicine
TopicBlood groups and transfusion
Canadian institutionsHéma-Québec
Fundersnot available
KeywordsGenotypingAmpliconAntigengenomic DNASerologyBlood group antigensGenotypePolymerase chain reactionComputational biologyBiologyImmunologyGeneAntibodyGenetics

Abstract

fetched live from OpenAlex

During the last decade, blood bank specialists have shown an increased interest in molecular analyses to complement serology work in determining blood group antigens. To efficiently respond to the numerous demands made for hemolytic disease of the newborn cases and polytransfused patients, we designed an inexpensive colorimetric high-throughput method to genotype several blood group antigens rapidly. Three simple steps are required to perform this technique: genomic DNA extraction, PCR amplification, and amplicon detection by a microplate ELISA. The 96-well plate format facilitates the manipulations and enables the analysis of multiple samples at once or the analysis of multiple antigens for fewer samples.The most common and clinically relevant minor blood group antigens were adapted to this method and are described in this work: Rh (D, C, c, E, e), Kell (K, k), Duffy (Fy(a), Fy(b)), and Kidd (Jk(a), Jk(b)). Other blood group antigens could be easily tested this way as long as their molecular basis is well established.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Other design · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.978
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0030.001
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.062
GPT teacher head0.451
Teacher spread0.390 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designOther design
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2009
Admission routes1
Has abstractyes

Explore more

Same venueMethods in molecular biologySame topicBlood groups and transfusionFrench-language works237,207