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Record W2412186354 · doi:10.1007/978-1-59745-471-1_19

Creation and Use of a Cre Recombinase Transgenic Database

2009· review· en· W2412186354 on OpenAlexaff
András Nagy, Lynn Mar, Graham Watts

Bibliographic record

VenueMethods in molecular biology · 2009
Typereview
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCRISPR and Genetic Engineering
Canadian institutionsLunenfeld-Tanenbaum Research InstituteMount Sinai Hospital
Fundersnot available
KeywordsRecombinaseDatabaseComputer scienceTransgeneComputational biologyBiologyGeneticsGeneRecombination

Abstract

fetched live from OpenAlex

In many cases, a gene "knockout" results in early embryonic lethality, which obscures the study of potential later functions. In other cases, the "knockout" does not show any phenotype due to the compensation of the gene deficiency by other family members. These limitations have called for further development of the powerful gene-targeting technology. One of the critical tools now being efficiently combined with gene-targeting is site-specific recombination. As the site-specific recombinase technology developed further in the mouse system, it became evident that this tool was going to have a significant impact on the power of mammalian genetics. The number of transgenic mouse lines expressing Cre recombinase with different specificities has steadily increased in the past 15 years and has now surpassed 500. Efficient utilization of this community-generated resource calls for a user-friendly database with all necessary information available about the properties of the Cre transgenic lines. The "CreXmice" database was created to meet these needs and has evolved over the past 4 years from flat file listings of transgenic lines into its current form, a professionally hosted SQL-driven web application. With hundreds of transgenic mouse lines, CreXmice is enriched by its presence on the World Wide Web allowing visitors the opportunity to search or contribute to the global effort by submitting the specific lines being developed by their laboratories.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: none
Teacher disagreement score0.013
Threshold uncertainty score0.045

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.003
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0020.001
Bibliometrics0.0050.006
Science and technology studies0.0010.001
Scholarly communication0.0030.003
Open science0.0040.002
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0130.026

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.051
GPT teacher head0.488
Teacher spread0.437 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations106
Published2009
Admission routes1
Has abstractyes

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