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Record W2416588127 · doi:10.1385/0-89603-487-9:211

Membrane Targeting via Protein N-Myristoylation

2003· article· en· W2416588127 on OpenAlexfundno aff
R.A. Jeffrey Mcllhinney

Bibliographic record

VenueHumana Press eBooks · 2003
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicProtein Kinase Regulation and GTPase Signaling
Canadian institutionsnot available
FundersPetroleum Technology Research Centre
KeywordsMyristic acidMyristoylationBiochemistryAmino acidFatty acidPeptide sequenceEnzymeAcyltransferasesChemistryBiologyPalmitic acidMembraneBiosynthesis

Abstract

fetched live from OpenAlex

The covalent modification of cell proteins by the attachment of myristic acid, a 14 carbon saturated fatty acid, to their N-terminal amino acid is now recognized to be a widespread phenomenon (1–5). The enzyme responsible for the attachment of myristic acid to these proteins is myristoyl-CoA: Protein N-myristoyltransferase (NMT; EC 2.3.1.97). This enzyme catalyzes the transfer of myristic acid from myristoyl-CoA to the N-terminal amino acid of the target protein and results in the fatty acid being amide-bonded to the α-amino group of the amino acid. Because this transfer is susceptible to protein synthesis inhibitors, it must take place as the protein is synthesized (6,7). Data from the studies on both the sequence of known myristoylated proteins, and from those on the sequence requirements for NMT, suggests that the N-terminal amino acid is always a glycine residue (5). However the other sequence requirements for protein N-myristoylation are less certain and the resulting vague general consensus sequence is summarized in Fig. 1. Although the majority of proteins that are substrates for NMT are myristoylated, the enzyme can make use of a limited range of other fatty acids, including shorter-chain and unsaturated fatty acids. In general, this occurs when these fatty acids form the majority of the acyl-CoA pool in a cell or tissue, for example, in the retina (8). For most practical purposes, however, NMT can be regarded as essentially specific for myristic acid.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.351
Threshold uncertainty score0.562

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.245
Teacher spread0.223 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations22
Published2003
Admission routes1
Has abstractyes

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