Elicitation effects of synthetic 1,2,4,5-tetraoxane and 2,5-diphenyltiophene in shoot cultures of two Nepeta species
Bibliographic record
Abstract
The present study was aimed to investigate the elicitation possibility of the main secondary metabolites production in Nepeta cataria L. and N. pannonica L. plants, by exposing them to synthetic compounds from the group of tetraoxanes and tiophenes. The effect of DO63 (1,2,4,5-tetraoxane) and DOVF15 (2,5-diphenyl-tiophene) on the production of cis,trans-nepetalactone (NL) and rosmarinic acid (RA) in two Nepeta species, was investigated in shoots grown on culture medium with addition of synthetic compounds in concentrations ranging from 0.1 to 2 mg L-1. The content of targeted metabolites in tested in vitro shoots depended on the type and the concentration of applied synthetic compounds. Application of DO63, primarily in concentration of 0.1 mg L-1 to 1 mg L-1, affected only NL production in both Nepeta species resulting in increased NL content in treated shoots, while production of RA was not influenced. Addition of DOVF15 caused decrease of RA content in N. pannonica shoots and increase in N. cataria shoots, whereas NL production was not affected. The presented results highlight the possibility of DO63 and DOVF15 application for the elicitation of the main secondary metabolites production in species from the genus Nepeta.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".