Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens
Bibliographic record
Abstract
Expression of the virulence genes of Agrobacterium tumefaciens occurs in acidic media containing sugars and certain phenolics secreted at plant wounds. In some strains of A. tumefaciens, exposure to these inducing conditions leads to inhibition of bacterial growth, which can then be followed by the selection of avirulent mutants (). Observation of this effect of vir gene induction depends on the use of particular experimental conditions. This chapter first specifies experimental parameters allowing to reveal, in A. tumefaciens, the modifications that accompany vir gene induction. Other parts of the chapter then describe how the mutants that are generated in the presence of acetosyringone are recognized and characterized. These protocols may prove useful to those with an interest in Agrobacterium biology. In addition, acetosyringone enhances the ability of A. tumefaciens to transform recalcitrant host plants, and as such has been incorporated in medium used for co-cultivation of bacteria and plant tissue (, , , ) or alternatively has been used to precondition explants prior to addition of bacteria (). Hence, the possibility of side effects of acetosyringone on A. tumefaciens bears direct relevance to experiments dealing with transformation of crop plants.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".