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Record W2467432455 · doi:10.1385/0-89603-408-9:187

Interaction of Cationic Peptides with Bacterial Membranes

2003· article· en· W2467432455 on OpenAlex
Shafique Fidai, Susan Walker Farmer, Robert E. W. Hancock

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueHumana Press eBooks · 2003
Typearticle
Languageen
FieldImmunology and Microbiology
TopicAntimicrobial Peptides and Activities
Canadian institutionsUniversity of British Columbia
FundersCystic Fibrosis CanadaCystic Fibrosis Foundation
KeywordsMelittinMembraneCardiolipinCytoplasmChemistryBiochemistryPeptideCecropinBiophysicsAmphiphileAntimicrobial peptidesBiologyPhospholipidOrganic chemistry

Abstract

fetched live from OpenAlex

A common feature of cationic peptides is that their site of action is at the membrane due to channel formation, and that they tend to possess strong selectivity towards then target membrane. For example, although moth cecropin and bee melittin are members of the same family of peptides that adopt amphipathic α-helical structures, the cecropins are strongly antibacterial and demonstrate minimal eukaryotic selectivity (i.e., toxicity), whereas melittin is a weak antibacterial compound but a potent toxin. Whereas the basis for selectivity is not completely understood, it has been shown to be due to the size of the transmembrane electrical potential gradient (up to −140 mV in bacterial cytoplasmic membranes compared with about −20 mV or less in eukaryotic membranes) and the lipid composition (bacterial membranes contain a large number of anionic lipids such as phosphatidyl glycerol and cardiolipin and lack cholesterol in their membranes). Gram-negative bacteria have an additional, outer membrane, and our data suggests that a further level of selectivity is expressed there in that there are Gram-positive bacteria-selective peptides that interact poorly with the outer membrane but (presumably) well with cytoplasmic membranes, whereas we have identified peptides that interact with the outer membrane, but are not bactericidal and thus do not interact with cytoplasmic membranes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.120
Threshold uncertainty score0.359

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.029
GPT teacher head0.239
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it