Molecular Characterization of a Catalase Gene (VsCat) from Vicia sativa
Bibliographic record
Abstract
In plants, catalases are encoded by a multigene family and are predominantly localized in the peroxisomes and glyoxysomes for scavenging H<sub>2</sub>O<sub>2</sub>. A full-length cDNA encoding <em>Catalase</em> (<em>VsCat</em>) was isolated from <em>Vicia sativa</em> using RT-PCR. The cDNA consisted of 1485 bp open reading frame (ORF) encoding a 494 amino acid polypeptide with a predicted molecular mass of 57.03 kDa and an estimated pI of 6.56. The predicted protein was highly homologues to other catalases from legume plants and contained typical catalytic active site, calmodulin binding domain, and internal peroxisomal targeting signal. Phylogenetic analysis revealed that VsCat is evolutionary close to faba bean and pea Cat1. The expression patterns of <em>VsCat</em> under different abiotic stresses and exogenous phytohormones were determined by quantitative RT-PCR<strong>. </strong>Compared to control plants, <em>VsCat</em> was differentially up-regulated in response to abiotic stresses and phytohormones. The expression analysis suggested that <em>VsCat</em> is involved in different abiotic stress responses. In future experiment, transgenic plants overexpressing <em>VsCat</em> might be a good choice to increase tolerance of forage plants to environmental stresses.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".