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Record W2498618443 · doi:10.1385/0-89603-077-6:1

Automated Amino Acid Analysis

2003· book-chapter· en· W2498618443 on OpenAlexaff
John A. Sturman, Derek A. Applegarth

Bibliographic record

VenueAmino Acids · 2003
Typebook-chapter
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBiopolymer Synthesis and Applications
Canadian institutionsBC Children's Hospital
Fundersnot available
KeywordsNinhydrinAmino acidReagentChemistryChromatographyPaper chromatographyHydrolysisAmino acid analysisAcid hydrolysisElutionOrganic chemistryBiochemistry

Abstract

fetched live from OpenAlex

Knowledge of the existence of amino acids dates back over a century in many cases, as does knowledge of their existence in proteins (see ). When amino acids were discovered, their identity was established by isolating and purifying the individual compounds and obtaining elemental analyses After the advent of paper chromatography, this technique was used with a variety of different solvents to identify elution characteristics and demonstrate the purity of isolated compounds. Amino acids were located by the use of a reagent that produced a color with the compound. The most common reagent used for locating amino acids is ninhydrin, which produces a purple color with amino acids, a pink or yellor color with amino acids, and various intermediate colors with compounds containing an amino group and a sulfonic acid, and so on. It also reacts with small peptides such as glutathione. The techniques of paper chromatography were applied to the separation of mixtures of amino acids, such as the components of a protein after hydrolysis, and then to the separation of free amino acids in physiologic fluids and tissues. It was extended by the use of two-dimensional chromatography, in which a different solvent was used in each direction Later, electrophoresis was employed as one of the separating techniques

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.031
Threshold uncertainty score0.103

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0040.003
Science and technology studies0.0010.000
Scholarly communication0.0020.001
Open science0.0020.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0310.024

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.240
Teacher spread0.229 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations9
Published2003
Admission routes1
Has abstractyes

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