Planarian Regeneration in Response to Drug Disruption of the Wnt and MAPK Pathways
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Bibliographic record
Abstract
The regenerative ability of planarians depends largely on its complex signaling pathways. The Wnt pathway regulates the A/P polarity formation after regeneration, while the MAPK pathway plays a role in regeneration. This experiment uses various drugs to disrupt the aforementioned pathways. Imatinib targets the RTKs, which play a role in the Wnt pathway. PZQ is expected to affect the Wnt noncanonical calcium pathway. EHT 1864 inhibits Rac1, a GTPase involved in the noncanonical PCP pathway. Finally, U0126 disrupts the MAPK pathway whose activity induces blastemic cell differentiation. After drug treatment, abnormal planarian regeneration is expected. The drug assays showed that while both Imatinib and PZQ have no effect on planarian regeneration, EHT 1864 under high concentration has a potent effect on the viability of planarians during regeneration. Furthermore, U0126 caused cyclopia in planarians under high concentrations. These observations suggest that the RTKs play a limited role in planarian regeneration, Rac1 plays a greater role than just A/P determination during regeneration, and that U0126 affects eye and head regeneration. Our findings also show an inconsistency with another research group’s study regarding the effects of PZQ on planarian polarity formation. L’abilité regénérative des planaires dépend largement sur la complexité des ses voies de signalisation. La voie des Wnt contrôle la formation de la polarité des potentiels d’action après la regénération, alors que la voie de la MAPK joue un rôle dans la regénération. L’imatinib cible les RTK, eux-mêmes jouant un rôle dans la voie des Wnt. Praziquantel est attendu d’affecter la voie de calcium non canonique des Wnt. L’EHT 1864 inhibe la Rac1, un GTPase impliqué dans la voie non canonique du PCP. Finalement, U0126 perturbe la voie de la MAPK, l’activité de laquelle induit la différentiation des cellules souches blastémiques. Après traitement avec de la drogue, de la regénération anormale des planaires est attendue. Les essais des drogues ont démontré que, bien que Imatinib et PZQ n’ont pas d’effets sur la regénération des planaires, l’EHT 1864 en haute concentration a un effet potent sur la viabilité des planaires durant la regénération. De plus, l’U0126 a causé la cyclopie chez les planaires en haute concentration. Ces observations suggère que les RTK jouent un rôle limité dans la regénération de planaires, la Rac1 joue un rôle plus important que simplement déterminer des potentiels d’action durant la regénération et l’U0126 affecte les regénérations des yeux et de la tête. Nos découvertes indiquent aussi des incohérences avec une étude par un autre groupe au sujet des effets du PZQ sur la formation polaire des planaires.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it