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Record W2523031282 · doi:10.1101/076158

Heterozygous mutation to <i>Chd8</i> causes macrocephaly and widespread alteration of neurodevelopmental transcriptional networks in mouse

2016· preprint· en· W2523031282 on OpenAlexaff
Andrea Gompers, Linda Su-Feher, Jacob Ellegood, Tyler W. Stradleigh, Iva Zdilar, Nycole A. Copping, Michael C. Pride, Melanie D. Schaffler, M. Asrafuzzaman Riyadh, Gaurav Kaushik, Brandon J. Mannion, Ingrid Plajzer-Frick, Veena Afzal, Axel Visel, L Pennacchio, Diane E. Dickel, Jason P. Lerch, Jacqueline N. Crawley, Konstantinos S. Zarbalis, Jill L. Silverman, Alex S. Nord

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2016
Typepreprint
Languageen
FieldNeuroscience
TopicAutism Spectrum Disorder Research
Canadian institutionsHospital for Sick Children
FundersLawrence Berkeley National LaboratoryIntellectual and Developmental Disabilities Research CenterU.S. Department of EnergyUniversity of California, DavisNational Institutes of HealthMIND Institute, University of California, DavisUniversity of California Berkeley
KeywordsMacrocephalyBiologyNeurogenesisAutismNeurodevelopmental disorderChromatin remodelingChromatinNeuroscienceRNA splicingBrain sizeGeneGeneticsMedicineRNA

Abstract

fetched live from OpenAlex

Summary The chromatin remodeling gene CHD8 represents a central node in early neurodevelopmental gene networks implicated in autism. We examined the impact of heterozygous germline Chd8 mutation on neurodevelopment in mice. Network analysis of neurodevelopmental gene expression revealed subtle yet strongly significant widespread transcriptional changes in Chd8 +/− mice across autism-relevant networks from neurogenesis to synapse function. Chd8 +/− expression signatures included enrichment of RNA processing genes and a Chd8-regulated module featuring altered transcription of chromatin remodeling, splicing, and cell cycle genes. Chd8 +/− mice exhibited increased proliferation during brain development and neonatal increase in cortical length and volume. Structural MRI confirmed regional brain volume increase in adult Chd8 +/− mice, consistent with clinical macrocephaly. Adult Chd8 +/− mice displayed normal social interactions, and repetitive behaviors were not evident. Our results show that Chd8 +/− mice exhibit neurodevelopmental changes paralleling CHD8 +/− humans and show that Chd8 is a global genomic regulator of pathways disrupted in neurodevelopmental disorders.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.013

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0040.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.248
Teacher spread0.228 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2016
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)→Same topicAutism Spectrum Disorder Research→French-language works237,207→