Cloning and expression of fusion (F) protein gene of Newcastle disease virus (NDV)
Bibliographic record
Abstract
The present study was undertaken to amplify, clone and express Fusion (F) protein gene of Newcastle disease virus. The freeze-dried virus of pigeon origin was propagated in embryonated chicken eggs. RNA was isolated from the infected allantoic fluid and cDNA was synthesized by reverse transcriptase polymerase chain reaction (RT-PCR). Primers specific to F gene incorporated with restriction enzyme sites, viz. NdeI and XhoI were used to amplify the F gene using cDNA as a template by polymerase chain reaction (PCR). The 1680 bp F gene amplicons were gel-purified and first cloned into TOPO vector. Positive clone was confirmed by colony PCR and restriction enzyme digestion. TOPO released insert was ligated with NdeI and XhoI digested pET22b expression vector using T4 DNA ligase and transformed into E.coli. Recombinant clones were selected by colony PCR and restriction enzyme digestion and induced with 0.5 mM final concentration of Isopropyl-1-thio-b-D-galactosidase (IPTG) for the expression of the recombinant F protein. The expressed protein of 55 kDa was obtained during the 4 h post-induction. The obtained recombinant protein reacted with rabbit anti serum in Western blot confirming it to be NDV specific.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".