P3‐185: Klotho Level, Redox Pattern and Oxidative Damages in Plasma from MCI and Alzheimer’s Patients
Bibliographic record
Abstract
The production of reactive oxygen species (ROS) leading to oxidative damage and neuronal cell death plays an early and important role in the pathogenesis of Alzheimer’s disease (AD). This study aimed to determine the total antioxidant capacity, the levels of reduced thiols, Klotho and protein carbonyls in plasma from mild cognitive impairment (MCI), and in AD patients at different stages of the disease. We have measured these different markers in plasma from patients at each disease stage and from age-matched controls (n=10). The total antioxidant capacity (TAC) was measured by electrochemical detection with the Free Radical analyzer Apollo 4000, total reduced protein thiols were quantified with the Ellman’s reagent (5,5′ - dithiobis-[2-nitrobenzoic acid], DTNB), Klotho level was evaluated using a sandwich enzyme-linked immunosorbent assay kit (Human Klotho DuoSet ELISA kit, R&D Systems, Inc.) and protein carbonyls were analyzed by Western Blot. The TAC is not different between controls and MCI patients but significantly decreased in AD groups. Protein carbonyl levels were similar in controls and MCI patients but were increased progressively increased between mild, moderate and severe AD patients. Klotho level increased in MCI as well as in mild and moderate AD. Interestingly, protein carbonyls and reduced thiols levels are correlated with the clinical MoCA and MMSE scores. Moreover, there is a correlation between TAC and level of carbonyl proteins in plasma. Our results indicate that the antioxidant capacity decreases with the progression of the disease while oxidized proteins increases. Interestingly, these peripheral markers of oxidative stress are negatively correlated with clinical score. Support: Fondation INRS-Armand-Frappier, Chaire Louise André Charron pour la maladie d Alzheimer, INAF, Univ. Laval.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".