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Record W2540224525 · doi:10.25560/9533

Regeneration of the heart with gene and cell therapy

2012· dissertation· en· W2540224525 on OpenAlexfundno aff
Amanda Claire Green

Bibliographic record

VenueSpiral (Imperial College London) · 2012
Typedissertation
Languageen
FieldMedicine
TopicTissue Engineering and Regenerative Medicine
Canadian institutionsnot available
FundersCanadian Cardiovascular Society
KeywordsRegeneration (biology)Genetic enhancementMedicineGeneBiologyComputational biologyGenetics

Abstract

fetched live from OpenAlex

Cardiovascular disease is the largest cause of morbidity and mortality in the industrialised world. Many patients with a longstanding history of coronary artery disease, multiple coronary interventions and a number of prior coronary events are no longer suitable for conventional revascularisation strategies and are often referred to as "no-option" patients. Despite intensive medical treatment, many present with clinical signs of heart failure and/or angina in the presence of underperfused, viable myocardium. I investigated two new strategies proposed to treat this patient population; angiogenesis induced by gene therapy in a pre-clinical model of myocardial ischaemia and regenerative therapy with autologous bone marrow derived putative stem cells in a Phase IIA pilot clinical trial. After establishing the ameroid porcine model of myocardial ischaemia, the efficacy of epicardially delivered Hypoxia Inducible Factor-1 alpha (HIF-1) was assessed with adenovirus (AdHIF-1α) and two plasmid constructs. AdHIF-1α was found to significantly increase myocardial blood flow and left ventricular function in this model. In a further study electromechanical mapping of ischaemic territory and endocardial delivery with AdHIF-1α demonstrated similar trends. I present the first clinical trial to demonstrate the feasibility of retrograde coronary venous delivery of putative stem cells to the heart. The inclusion of 111-In oxide labelled cells demonstrated a sustained myocardial signal up to 21 days. Retention of cells within the myocardium is likely to be critical in allowing a biologically important paracrine function or stem cell differentiation. In the six-month follow-up, there were no major safety concerns, although only hypothesis generating efficacy endpoints were included, significant improvements were seen in Quality of life, New York Heart Association (NYHA) class and myocardial oxygen consumption (MVO2). A positive correlation was seen with the number of CD34+ cells delivered and change in MVO2. Further randomised placebo controlled trials are required to elucidate further any perceived improvements in efficacy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.052
Threshold uncertainty score0.747

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.238
Teacher spread0.227 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2012
Admission routes1
Has abstractyes

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