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Record W2565447503

Predicting the Relative Sensitivity of Sturgeons to Aryl Hydrocarbon Receptor Agonists

2016· dissertation· en· W2565447503 on OpenAlexfundno aff
Jonathon A Doering

Bibliographic record

VenueUniversity Library - University of Saskatchewan (University of Saskatchewan) · 2016
Typedissertation
Languageen
FieldAgricultural and Biological Sciences
TopicPharmacological Effects and Assays
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsAryl hydrocarbon receptorSensitivity (control systems)ArylChemistryHydrocarbonOrganic chemistryBiochemistryEngineeringAlkylGene
DOInot available

Abstract

fetched live from OpenAlex

Along with overexploitation and habitat loss, pollution is one cause for decreases in populations of fishes.One class of pollutants of particular global environmental concern to fishes are dioxinlike compounds (DLCs).DLCs elicit their toxicity through activation of the aryl hydrocarbon receptor (AHR).Despite this common mechanism of all DLCs, dramatic differences in sensitivity exist among fishes.Sturgeons (Acipenseridae) are an ancient family of fishes in which most species are endangered.It is hypothesized that pollutants, including DLCs, might be contributing to the observed declines in populations because sturgeons have a unique life-style that makes them susceptible to exposure to bioaccumulative chemicals.However, determining sensitivities of sturgeons to DLCs through traditional in vivo toxicity testing is not feasible for practical and ethical reasons.Therefore, the aim of this research was to develop a mechanismbased biological model capable of predicting the relative sensitivity of sturgeons to DLCs.This mechanism-based biological model was developed through investigations into the AHR and AHR-mediated molecular and biochemical responses of white sturgeon (Acipenser transmontanus) relative to teleost fishes and another species of sturgeon.White sturgeon responded to activation of the AHR in a manner that is consistent with responses of teleost fishes (induction of cytochrome P450 1A).Two AHRs with similar levels of expression were identified in white sturgeon, an AHR1 that resembles AHR1s of tetrapods and an AHR2 that resembles AHR2s of other fishes.Both AHR1 and AHR2 of white sturgeon were activated by exposure to five selected DLCs in vitro with effect concentrations less than any other AHR tested to date.These findings were suggestive that white sturgeon might be among the most sensitive species of fish to exposure to DLCs.These findings raised the question as to whether other members of the continuing to be a significant part of my time as a Ph.D. student and being a major influence on my growth as a scientist.Also special thanks to my Ph.D. supervisor Dr.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Qualitative · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.700
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0000.001
Science and technology studies0.0010.001
Scholarly communication0.0000.001
Open science0.0020.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.175
Teacher spread0.168 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designQualitative
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2016
Admission routes1
Has abstractyes

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