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Manipulating Stem Cells for Therapeutic Expansion.

2009· article· en· W2581803614 on OpenAlexaff
Guy Sauvageau

Bibliographic record

VenueBlood · 2009
Typearticle
Languageen
FieldMedicine
TopicMesenchymal stem cell research
Canadian institutionsUniversité de MontréalInstitute for Research in Immunology and Cancer
Fundersnot available
KeywordsStem cellHaematopoiesisTransplantationBiologyHematopoietic stem cellImmunologyCancer researchFusion proteinHematopoietic stem cell transplantationEx vivoCord bloodIn vivoMedicineCell biologyRecombinant DNAInternal medicineGeneGenetics

Abstract

fetched live from OpenAlex

Abstract Abstract SCI-42 “Self-renewal” is a process central to the expansion of normal and cancerous stem cells and its understanding is critical for future advances in transplantation-based therapies and cancer treatment. Even today, many patients are deprived of the benefit of a successful blood stem cell transplant because the number of allogeneic or autologous stem cells available is insufficient, which results in delayed hematopoietic recovery post-transplant, or exclusion of a transplant-based therapeutic option altogether. The molecular machinery controlling self-renewal of hematopoietic stem cells (HSCs) remains poorly defined with the exception of a few genes such as HOX4 and Bmi1. We and others recently demonstrated the capacity of a recombinant HOXB4 protein (TAT-HOXB4 fusion protein) to stimulate mouse and human HSC self-renewal divisions in culture. Technical difficulties inherent to this recombinant protein have postponed the initiation of clinical trials. In part to overcome this hurdle, we have developed a novel in vitro/in vivo gain-of-function screen and identified several nuclear factors which expand hematopoietic stem cell ex vivo. A significant proportion of these factors display HOXB4-like properties and show non-cell autonomous activity. Initial results suggest that some of these new factors are also active with human cord blood derived HSCs. The generation of novel TAT fusion proteins will open new possibilities in the therapeutic expansion of human HSCs. Disclosures No relevant conflicts of interest to declare.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.007
Threshold uncertainty score0.024

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0070.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.078
GPT teacher head0.331
Teacher spread0.253 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

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