Salinity-regulated expression of genes involved in GABA metabolism and signaling
Bibliographic record
Abstract
4-Aminobutyrate (GABA) is a nonproteinogenic amino acid that functions in stress tolerance and signaling. Here, we report that salinity stress and elevated GABA levels coincided with the induction of glutamate decarboxylase 4 (GAD4) expression in Arabidopsis thaliana (L.) Heynh. ecotype Col-0. In-silico and microarray analysis revealed the over-representation of binding sites for WRKY and MYB transcription factors in the GAD4 promoter, as well as their co-expression with GAD4. Transcript profiling of liquid culture-grown, wild-type plantlets subjected to salinity stress for up to two days confirmed that GAD4 expression is associated with the inducible co-expression of WRKY28, WRKY30, WRKY40, MYB2, MYB15, and MYB108, as well as calmodulin-like 37 and aluminum-activated malate transporter 2, suggesting the involvement of gene regulation, protein activation, and anion transport in GABA accumulation. Transcript profiling of shoots from soil-grown, wild-type plants and corresponding single and double GAD mutants subjected to two days of salinity stress suggests that the GABA accumulation could involve post-translational activation of pre-existing GAD1 and GAD2 by elevated cytosolic calmodulin, as well as induction of GAD4 expression.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".