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Human and Murine Immune Thrombocytopenia (ITP) Is Associated with a Peripheral Deficiency of CD4- T Regulatory Cells (Tc-regs)

2012· article· en· W2585146168 on OpenAlexaff
John W. Semple, Daoxin Ma, Rukhsana Aslam, Ming Hou, Heyu Ni, Yu Hu

Bibliographic record

VenueBlood · 2012
Typearticle
Languageen
FieldMedicine
TopicPlatelet Disorders and Treatments
Canadian institutionsCanadian Blood ServicesSt. Michael's Hospital
Fundersnot available
KeywordsFOXP3IL-2 receptorCD8ImmunologyPopulationRegulatory T cellImmune systemFlow cytometryPeripheral tolerancePeripheral blood mononuclear cellT cellMedicineImmune toleranceBiologyIn vitro

Abstract

fetched live from OpenAlex

Abstract Abstract 3333 CD8+ T suppressor cells were originally described by Gershon on Kondo more than 40 years ago, however, because of difficulties in their characterization, they fell out of favor for decades. T suppression was resurrected by the discovery of CD4+ CD25+ FoxP3+ T regulatory cells as being the critical cell in the maintenance of self tolerance. Recently, a non-conventional CD4-CD25+FoxP3+ T cell subset termed Tc regulatory cells (Tc-regs) has been described that are also important in inducing tolerance against self. A peripheral CD4+ Treg deficiency has been suggested to be the potential cause of ITP, however, the role of this newly described CD4- T cell population has not been determined. To address the role of these cells, we studied lymphocyte responses in 36 patients with ITP (18 newly-diagnosed and 18 in remission) and a murine model of ITP. Peripheral blood mononuclear cells from patients or spleens and thymi from mice were isolated and FoxP3 expressing Tc-regs were evaluated by flow cytometry. Results show that compared with healthy control subjects, the percentages of peripheral Tc-regs in newly diagnosed patients with ITP were significantly decreased. The Tc-reg deficiency was rescued in those patients in remission due to therapy. Similar results were observed in an active model of murine ITP. Additionally, the murine model of ITP revealed that the CD4- deficient population expressed CD8. These results suggest that, like CD4+ T regs, there is a significant reduction in peripheral Tc-regs in both human and murine ITP and disease remission correlates with rescuing the deficiency. Disclosures: No relevant conflicts of interest to declare.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.238
Teacher spread0.227 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2012
Admission routes1
Has abstractyes

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