Optimizing Autologous Mononuclear Cell Collections for Cellular Therapy Using the Novel Optia Apheresis Device in Pediatric Patients with High Risk Leukemia
Bibliographic record
Abstract
Background: Manufacturing of cellular products for immunotherapy such as CAR T-cells requires successful collection of mononuclear cells (MNC). Collections from children with high risk leukemia present a challenge as the established COBE Spectra apheresis device (COBE) is being replaced by the novel Spectra Optia device (Optia) and published experience with the Optia device in children is lacking. Our aim was to compare the two collection devices, and describe settings to optimize MNC collections on the new Optia device. Study Design and Methods: As a quality initiative we retrospectively collected and compared data from MNC collections on both devices. Collected data included patient's demographic and clinical characteristics, collection parameters including pre collection lymphocyte and CD3+ counts, total blood volumes (TBVs) processed, procedure runtimes, side effects including CBC and electrolyte changes, and product characteristics including product volumes and cell counts. Collection efficiencies and collection ratios were calculated. Results: Twenty six MNC collections were performed on 20 pediatric patients; 11 with the COBE device and 15 with the Optia device. Adequate MNC products were successfully collected with a single procedure from all patients on both machines except the first collection on the Optia. The mean calculated MNC collection efficiency was significantly higher with Optia collections as compared to COBE (57.9 ± 4.6% vs 40.3 ± 6.2%, respectively, P = .04). CD3+ yields where comparable on both machines with mean product CD3+ of 2.03 ± .44 cells/kg with COBE vs 1.54 ± .24 cells/kg with Optia (P = .34). Significantly smaller blood volumes were processed with Optia with comparable collection times. Collected product volumes on the Optia were significantly larger and contained significantly less white blood cell and red blood cell counts. Recent chemotherapy did not affect collection yields. No significant side effects attributed to the procedure were noted. Conclusion: MNC apheresis using the Optia device in children is more efficient and as safe as with the COBE device.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".