Assessing Polymorphism Information Content (PIC) Using SSR Molecular Markers on Local Species of Citrullus Colocynthis. Case Study: Iran, Sistan-Balouchestan Province
Why this work is in the frame
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Bibliographic record
Abstract
Studying polymorphism information content in plants, particularly local plants which are rich in genetic variety, can play an efficient role in building genetic bank and species’ breeding. Iranian colocynth is highly important in terms of medicinal and therapeutic traits and hence, it needs assessing the polymorphism information content. In present research, simple sequence repeat (SSR) markers are used. 32 samples are randomly collected from local accumulations in 8 regions of Sistan-Balouchestan province. DNA extraction from each sample was done using Cetyltrimethylammonium Bromide. 10 primers were designed and used in this study. Polymerase chain reaction was done using extracted DNA and ten primers. To analyze genetic data, NTSYS pc ver.2/2, Genalex 6.5 and XLSATAT software were used. Regarding data analysis of respective values and taking this fact into account that7 out of 10 used primers showed polymorphism information content in respective samples, their classification was not possible and hence, categorization of similar shapes was not performed due to highly genetic diversity of this species and, also, each value had a different shape.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.004 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it