Effect of Gonadotropin Releasing Hormone Agonist-Buserelin on In Vitro Induction of Acrosome Reaction in Bovine Spermatozoa.
Bibliographic record
Abstract
Acrosome reaction (AR) is an important step in the process of fertilization in mammals which takes place in the oviduct. Recent studies suggest that Gonadotropin Releasing Hormone (GnRH) can act in an autocrine or paracrine manner in reproductive tissues and cells to modulate their physiological functions. It is also evident that functional forms of GnRH- GnRH receptor system could be present in the oviductal microenvironment. Thus this study was performed to assess the direct effect of GnRH on induction of AR in bovine spermatozoa. Frozen thawed bovine spermatozoa after swim up separation in modified Tyrodes medium were treated with a GnRH agonist, buserelin at the dose rates of 0 (control), 0.2, 0.4, 0.8, 1.2μgmL-1 and 1 μgmL-1 progesterone (positive control) and incubated at 38.50C in a humidified atmosphere of 5% CO2 for 3 hours. Acrosomal status at 0 hour and after incubation in each treatment group was determined with fluorescent microscopy, using fluorescein isothiocyanate labeled Pisum sativum agglutinin (FITC-PSA) staining. Increase in percent acrosome reacted after 3 hours were significantly higher (p<0.001) in the presence of 0.4 and 0.8 μgmL-1 when compared with the control group that was incubated in the absence of buserelin. While 0.2 and 1.2 μgmL-1 were also able to increase the percent acrosome reacted insignificantly (p >0.05).Increase in the percentage of acrosome reacted spermatozoa in different treatment groups were 24.115 ± 1.201, 29.65 ± 0.8685, 36.39 ± 2.165, 48.32 ±1.254, 27.05 ± 0.6074 and 34.836 ± 1.143 for 0, 0.2, 0.4, 0.8, 1.2μgmL-1 buserelin and 1 μgmL-1 progesterone respectively. In addition, it was also revealed that this stimulatory effect of GnRH agonist could be blocked by introduction of 1000ng mL-1 Antide (GnRH antagonist) in to the incubation medium, suggesting a receptor mediated stimulatory effect of GnRH agonist on bovine spermatozoa. The results of this study further strengthens our knowledge on the presence of GnRH-R system and the role of GnRH in bovine reproductive biology which would contribute to the more meaningful application of GnRH and its potent synthetic analogues, in both domestic animal and human reproductive management and in vitro fertilization programs.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".