Mining New Bt Insecticidal Protein Genes Resource Using Illumina Sequencing Technology
Bibliographic record
Abstract
Bacillus thuringiensis ( Bt ), as the most widely used biologically produced pest control agent, is already auseful alternative or supplement to synthetic chemical pesticide and is also a key source of genes for transgenic expression to provide pest resistance in plants. On basis of Bt strains isolated from soil samples from the Diaoluoshan and Wuzhishan Natural Reserve, we carried out a large-scale screening the insecticidal genes from the special Bt strains through Illumina sequencing. In this study, 25 of the insecticidal genes, including 5 of the novel vegetative insecticidal protein genes ( vip ) and 3 of the new cyt genes had been identified from the different Bt strains. All the result indicated that the second generation sequencing technology of illumine is efficient in mining the insecticidal gene from Bt isolates, which would further enrich the new different insecticide genes application in commercial agriculture, forest management, and mosquito control.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".