Characterization of the Cyanobacterial Harmful Algal Bloom Community in Hamilton Harbour, Lake Ontario
Bibliographic record
Abstract
Little is known about the interspecific relationships between Cyanobacteria and heterotrophic microorganisms throughout Cyanobacterial harmful algal bloom (CyanoHAB) development mainly since most aquatic microorganisms are non-culturable in the laboratory. The culture-independent technique Terminal Restriction Length Polymorphism (T-RFLP) was used for fingerprinting of microbial community DNA collected from Hamilton Harbour water throughout the summers of 2014 and 2015. 454-tag encoded pyrosequencing and shotgun sequencing revealed the dominant prokaryotes and eukaryotes throughout each summer. Actinobacteria was the dominant bacterial phylum in June while Cyanobacteria and Proteobacteria were co-dominant from August-September. Shotgun data also revealed the microbial functional potentials. Cyanobacterial dominance was associated with a higher potential for nitrate/nitrite assimilation and nitrogen fixation in the late-summer. These results provide the first metagenomic characterization of the total CyanoHAB community in Hamilton Harbour. Correlations to physico-chemical parameters further provide insights for future management into which factors could be shaping the bacterial community throughout the summer.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.027 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".