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Record W2607174738

Evolution of a Bacterial Global Regulator- Lrp

2016· article· en· W2607174738 on OpenAlexfundno aff
Yvette Unoarumhi

Bibliographic record

VenueOhioLink ETD Center (Ohio Library and Information Network) · 2016
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrobial Metabolic Engineering and Bioproduction
Canadian institutionsnot available
FundersCollege of Medicine and Life Sciences, University of ToledoMcMaster University
KeywordsRegulatorBiologyGeneticsGene
DOInot available

Abstract

fetched live from OpenAlex

Global regulators each control hundreds of genes in bacteria, and it is still unclear how these regulators evolve, especially considering that gene regulation changes more rapidly than the regulated genes themselves.Leucine-responsive regulatory protein (Lrp) is a global regulator in enteric bacteria, controlling both metabolic and virulenceassociated genes.Lrp orthologs are found among both Bacteria and Archaea.Surprisingly, even within the phylum γ-Proteobacteria, Lrp is a global regulator in some orders and a local regulator in others.This raises important questions about the evolution of Lrp functions.The way global regulators function is crucially important to bacterial physiology.This thesis presents studies on the evolution and regulation pattern of Lrp, carried out with the goal of providing insights into global regulators more generally.Two independent studies of Lrp were carried out.The first compared Lrp sequences from four bacterial orders within the γ-Proteobacteria: Enterobacteriales, Vibrionales, Pasteurelalles, and Alteromonadales.AsnC was also analyzed in parallel for comparison, as it is a paralog of Lrp that in all known cases is a local regulator controlling a small number of genes.As expected, Lrp and AsnC sequences formed two

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0010.002
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0030.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.003
GPT teacher head0.175
Teacher spread0.171 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2016
Admission routes1
Has abstractno

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