The enhancer of zeste gene OsiEZ1 is involved in ligule and seed development in rice
Bibliographic record
Abstract
The epigenetic control of gene expression by histone methylation influences plant growth and development. OsiEZ1, encoding a putative histone methyltransferase for trimethylation of histone H3 lysine 27 (H3K27me3), was recently shown to be involved in the photoperiodic control of rice flowering. However, to date, the role of OsiEZ1 in organ development has not been characterized in detail. Here, we show that the loss of OsiEZ1 results in abnormal ligule and seed development. Morphological analysis showed that the ligules in flag leaves of the T-DNA insertion mutant osiez1 were shorter than those of the wild-type, whereas plant height and flowering time in the mutant were similar to those of the wild-type. In addition, the seed size and weight of the osiez1 mutant were reduced compared with those of the wild-type. Expression analysis showed that the transcript levels of the GRAIN WIDTH 2 (GW2) gene and chaperone protein gene PROTEIN DISULFIDE ISOMERASE-LIKE 1-1 (PDIL1-1) were reduced in the osiez1 mutant compared with the wild-type during seed development, while the expression levels of seed storage protein genes Glb and Gt were unchanged in the osiez1 mutant. These results suggest that OsiEZ1 may play important roles in ligule and seed development.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".