Plasmids containing the same origin of replication are useful tools to perform biotechnological studies in Pseudomonas putida U and in E. coli DH10B
Why this work is in the frame
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Bibliographic record
Abstract
Plasmids containing the same origin of replication (pBBR1MCS-2 KmR and pBBR1MCS-3 TcR) have been used to express simultaneous and independently different proteins in P. putida U and in E. coli. Thus, when P. putida was transformed with different genetic constructions made in the same plasmid (pBBR1MCS-3 TcR), or with plasmids containing the same replication origin but with different antibiotic resistant genes (KmR and TcR), they co-existed inside the same microbe. Furthermore, when E. coli DH10B was transformed with the plasmids recovered from the recombinant P. putida U, we noticed that all the bacteria isolated from single colonies are resistant to Km and Tc, suggesting that these plasmids were also present in E. coli. This observation facilitates the genetic manipulation of these strains (i.e. avoiding the use of different plasmids in double or multiple complementation experiments), and could be an interesting tool to approach many metabolic and biotechnological studies.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it