Successive outermost-to-core shell directionality of the protonation of poly(propyl ether imine) dendritic gene delivery vectors
Bibliographic record
Abstract
The protonation behaviour of polycationic compounds has direct relevance to their ability to condense and deliver nucleic acids. This report pertains to a study of the protonation behaviour of polycationic poly(propyl ether imine) (PETIM) dendritic gene delivery vectors that are constituted with tertiary amine core moiety and branch sites, n-propyl ether linkages, and primary amine peripheries. The ability of this series of dendrimers to condense nucleic acids and mediate endosomal escape was studied by unravelling the protonation behaviour of the dendrimers aided by pH metric titrations and 1H and 15N NMR spectroscopies. The results demonstrate protonation of the primary and tertiary amines of outermost-to-core shells occurring in a successive stepwise fashion, in contrast to other polycationic vectors. Theoretical calculations based on the Ising model rationalize further the finer details of protonation at each shell. The protonation pattern correlates with the endosomal buffering and nucleic acid condensation properties of this PETIM-based dendritic gene delivery vectors. The study establishes that the protonation behaviour is a critical and essential parameter to assess the gene condensation and delivery vector properties of a polycationic compound.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".