437 Effects of lipopolysaccharide challenge and weaning on serum biochemical parameters and hepatic hepcidin gene expression in piglets
Bibliographic record
Abstract
Hepcidin is a liver-derived peptide hormone that acts as a key regulator of intestinal iron absorption with antimicrobial properties in mammals, and its expression can be regulated by multiple stimuli. Two experiments were conducted to investigate the effects of lipopolysaccharide (LPS) challenge and weaning on serum parameters and hepatic hepcidin gene expression in piglets. In Exp. 1, 10 suckling piglets were randomly divided into a control group and a LPS group, with 5 piglets per group. The LPS group pigs were injected with 100 μg/kg BW LPS on suckling d 3, 5, and 7, and the control group piglets were injected with normal saline at the same dose. All piglets were sacrificed on d 7, and blood, liver, and spleen samples were collected. Total iron contents in the liver and serum were decreased (P < 0.05) in the LPS group. Serum total protein, albumin, total bilirubin, glucose, and triglyceride levels were reduced (P < 0.05) in the LPS group. Activities of serum Cu/Zn superoxide dismutase, catalase, and phospholipid hydroperoxide glutathione peroxidase were also decreased (P < 0.05) in the LPS group. However, erythrocyte malondialdehyde content was increased (P < 0.05) by the LPS challenge. Hepatic hepcidin mRNA abundance was increased (P < 0.05) in the LPS group compared with the control group. In Exp. 2, 55 piglets were randomly taken from 5 different sows at 21 d of age, and 5 piglets were sacrificed right before weaning at the age of 21 d. The remaining 50 piglets were weaned and divided into 5 pens with 10 pigs per pen. The weaning pigs were fed a corn and soybean meal–based weaning diet, and 1 pig per pen was sacrificed on d 2, 4, and d 8 after weaning. The alkaline phosphatase (AP) activity in serum was highest right before weaning and decreased (P < 0.05) after weaning. Serum Na, Cl, and K ion levels were not different (P > 0.05) before and after weaning. Serum globulin content was reduced (P < 0.05) on d 2 and was increased (P < 0.05) on d 4 and 8 after weaning. The hepatic hepcidin mRNA abundance was the lowest (P < 0.05) before weaning, peaked on d 2, and was then reduced (P < 0.05) on d 4 and 8 after weaning. In conclusion, LPS challenge and weaning enhanced hepatic hepcidin mRNA abundances whereas weaning decreased serum AP activity in the piglets.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".