P6047 Expression of TLR2 pattern recognition receptor on mononuclear cells cultured with ligands among cattle ranked by estimated breeding values for adaptive immune response traits
Bibliographic record
Abstract
Toll-like receptor 2 (TLR2) is a pattern recognition receptor expressed on epithelial cells and some leukocytes. Binding of bacterial ligands to TLR2 induces proinflammatory innate responses and contributes to the development of adaptive immune responses. A study was conducted to investigate differences in expression of TLR2 on blood mononuclear cells (BMC) ex vivo, and in vitro following an 18-h culture with TLR2 ligands. Dairy cattle (N = 18) were classified into five phenotypic groups based on their estimated breeding values (EBV) for antibody-mediated (AMIR) and cell-mediated (CMIR) immune responses. The association of EBVs for AMIR and CMIR (as categorical variables) and TLR2 expression on BMC was evaluated. Cattle that were greater than one standard deviation above the mean EBVs for AMIR or CMIR were classified as High (H), those more than one standard deviation below were classified as Low (L), and responses in between were classified as Average (A). Phenotypic groups studied were as follows: L-AMIR/L-CMIR, L-AMIR/H-CMIR, A-AMIR/A-CMIR, H-AMIR/L-CMIR and H-AMIR/H-CMIR. Ligands tested included lipotechoic acid from S. aureus (LTA), mannose-capped lipoarabinomannan from H37Rv M. tuberculosis (Man-LAM), and PAM3CSK4 (PAM, a tri-acylated synthetic lipoprotein). Mononuclear cells were stained using monoclonal antibodies (clones HCA152F, MM61A, BAQ155A, MM1A specific for TLR2, CD14, B cells, and CD3, respectively), and characterized using flow cytometry. Ex vivo, CD14+ mononuclear cells had the highest percentage of cells expressing TLR2 among all BMC. Among groups, H-AMIR/H-CMIR cows had a significantly higher median fluorescent intensity (MFI) of TLR2 expression compared with A-AMIR/A-CMIR cows on all BMC. In vitro, on all BMC, H-AMIR/H-CMIR had a significantly higher MFI of TLR2 expression compared with H-AMIR/L-CMIR following culture with PAM or LTA. For the CD14+ cell subset, MFI was highest for H-AMIR/H-CMIR group compared with L-AMIR/H-CMIR, A-AMIR/A-CMIR, and H-AMIR/L-CMIR cows following culture with PAM only. Although Man-LAM did influence the percentage of cell subsets expressing TLR2, no significant differences were noted among EBV groups. The higher expression of TLR2 on BMC among cattle that rank high for both AMIR and CMIR may contribute to enhanced adaptive immune responses and disease resistance.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".