Membrane Topology and Identification of Critical Amino Acid Residues in the Wzx O-Antigen Translocase from <i>Escherichia coli</i> O157:H7
Bibliographic record
Abstract
Volume 192, no. 23, p. 6160-6171, 2010. Page 6160: The title should appear as shown above. Page 6165: Fig. Fig.33 should appear as shown below. (The wzxEcO157 gene was obtained by PCR cloning from genomic DNA of strain UWO934-88, a clinical isolate of E. coli O157:H7 [Marolda et al., Microbiology 150:4095-4105, 2004], and its amino acid sequence is identical to that of GenBank accession no. AE005429_8. The amino acid sequence of WzxEcO157 includes an N-terminal fusion to the FLAG epitope. To construct this fusion, the first Met of wild-type WzxEcO157 was changed to Gly [position 1/13]. The amino acid numbers in the figure correspond to the original positions in the wild-type protein [Gly-13 corresponds to Met-1]. The tyrosine residue at position 319 in the original figure was misplaced, and its normal location is at position 253. The methionine at position 405 was displaced graphically but is actually located at the end of cytosolic loop 5.) FIG. 3. Page 6168: Table Table33 should appear as shown below (column heads have been corrected). TABLE 3.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".