Abstract P215: Angiotensin II-Mediated Cardiac Remodeling in cChAT Mice
Bibliographic record
Abstract
It has been demonstrated that the cardiac non-neuronal cholinergic system (NNCS) plays a role in regulating cardiac homeostasis under physiological conditions. To examine a possible role played by NNCS upon cardiac remodeling, we submitted mice with genetic deletion of cardiomyocyte-specific choline acetyltransferase (cChAT) to chronic subcutaneous infusion of angiotensin II (Ang II). Male mice aged six months were assigned into four experimental groups: wild-type (WT) + Saline; WT + Ang II; cChAT + Saline and cChAT + Ang II. Ang II did not change the heart weight in WT mice (10.4±0.3 vs. 8.5±0.2 mg/mm in WT+Saline) but determined an increase in cChAT mice (12.8±1 vs. 8.8±0.6 mg/mm in cChAT+Saline). In WT mice, Ang II decreased left ventricular (LV) fractional shortening (23±2 vs. 35±2 % in WT+Saline) and LV ejection fraction (54±4 vs. 73±2 % in WT+Saline). However, cChAT+Ang II mice exhibited greater decrease in both LV fractional shortening (12±1.8 vs. 23±2 % in WT+Ang II) and ejection fraction (31±4 vs. 54±4 % in WT+Ang II). cChAT mice displayed cardiomyocyte hypertrophy (2002±116 vs. 1377±48 μm2 in WT+Saline) even when receiving saline. Ang II increased the cardiomyocyte surface area in both WT and cChAT mice; however, the cChAT mice exhibited greater myocyte hypertrophy (3035±181 vs. 2603±122 μm2 in WT+Ang II). Hematoxylin and eosin staining revealed that Ang II promoted greater disruption of myocardial structure in cChAT mice. Additionally, Trichrome C staining revealed that WT+Ang II mice presented increased collagen deposition (2.86±0.2 vs. 0.45±0.07 % in WT+Saline); nevertheless, the fibrotic response in cChAT+Ang II mice was greater than that observed in WT animals (6.16±1.1 vs. 2.86±0.2 % in WT+Ang II). Therefore, mice with deficiency for ChAT displayed exacerbated ventricular dysfunction induced by chronic Ang II administration, providing support for a role of the NNCS in the progression of cardiac remodeling.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.009 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".