Biological stoichiometry and bioenergetics of <i>Fusarium oxysporum</i> EKT01/02 proliferation using different substrates in cyanidation wastewater
Bibliographic record
Abstract
Abstract Cyanidation wastewater contains heavy metals, including high concentrations of ammonia and free cyanide (CN ‐ ). Aerobic growth of Fusarium oxysporum EKT01/02 in synthetic gold mine wastewater under different substrates was examined using biological stoichiometry and thermodynamic models in batch systems. The molecular weight of the dry biomass obtained was 23.03 g/C‐mol, 33.14 g C‐mol −1 , and 27.06 g/C‐mol in glucose with ammonia (GA), Beta vulgaris with ammonia (BA), and B. vulgaris with cyanide (BCN) cultures, respectively. The microbial growth model showed the highest biomass yield of 0.69 g dry cell/g substrate in BA cultures. The heat of reaction ( ) and Gibbs energy dissipation per mole of biomass formed ( ) were −652.55/−432.11 kJ/C‐mol, −132.59/−471.19 kJ/C‐mol, and −370.34/−225.35 kJ/C‐mol ‐ for GA, BA, and BCN cultures, respectively. The total Gibbs energy dissipated increased steadily over time and the metabolic rate of the F. oxysporum used was minimally adversely affected by the cyanidation wastewater as shown by the degree of reduction including the respiratory quotient quantified. The F. oxysporum proliferation was determined to be enthalpically driven in the cultures studied. This study revealed that the use of B. vulgaris agro‐waste for the bioremediation of cyanidation wastewater is feasible and could engender sustainability of gold mining wastewater treatment processes.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".