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Record W2736829225 · doi:10.1002/lno.10623

Phagocytosis of microbial symbionts balances the carbon and nitrogen budget for the deep‐water boreal sponge <i>Geodia barretti</i>

2017· article· en· W2736829225 on OpenAlexafffund
Sally P. Leys, A Kahn, James Kar‐Hei Fang, Tina Kutti, Raymond J. Bannister

Bibliographic record

VenueLimnology and Oceanography · 2017
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMarine Sponges and Natural Products
Canadian institutionsUniversity of Alberta
FundersNorges ForskningsrådUniversity of AlbertaHavforskningsinstituttet
KeywordsSpongeDetritusBiologyCarbon fibersNitrogenEnvironmental chemistryChemistryBotanyEcology

Abstract

fetched live from OpenAlex

Abstract Geodia barretti is a massive nearly spherical sponge that forms dense assemblages on the continental shelf of the North Atlantic and the Norwegian Sea. We studied the metabolism of individual sponges collected using a remotely operated vehicle and maintained in large tanks with high volumes of unfiltered water brought from 160 m depth. We used direct methods (In‐Ex) to measure excurrent flow rates, oxygen removed, and carbon and nutrient flux through the sponges. G. barretti had very low specific filtration (0.26 mL min −1 mL −1 sponge tissue) and low respiration (5.34 ± 0.98 nmol O 2 min −1 mL −1 sponge tissue; 8.44 ± 1.51 μmol O 2 h −1 g C −1 ) rates in comparison to other sponges. A net release of nitrogen was detected as . Bacteria were removed from the water filtered with up to 99% efficiency, yet comprised only 5% of the sponges′ total carbon budget; the remainder consisted of dissolved organic carbon and detritus. High bacterial removal was aided by the presence of a tight gasket of cells that surrounds the collar of each choanocyte filter. A test for potential bypass canals showed removal of fluorescent microspheres until they were excreted 5–12 h after feeding. Electron micrographs showed active uptake of Escherichia coli “fed” to the sponge as well as phagocytosis of symbiont microbes by sponge cells in the mesohyl. These data provide the first comprehensive study of metabolism in a deep‐water high microbial abundance sponge.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.471
Threshold uncertainty score0.329

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.219
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations79
Published2017
Admission routes2
Has abstractyes

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