Inspection of Phytochemical Content and In Vitro Antioxidant Profile of Gnaphalium luteoalbum L.: An Unexplored Phytomedicine
Bibliographic record
Abstract
Background:Oxidative stress is intensely linked with several pathological manifestations. Searching for medicinal plant with the superior safety profile for the treatment of oxidative stress related disorders are ongoing due to multiple unwanted effects associated with synthetic antioxidants. Therefore the purpose of this study was to examine the phytochemical content, in vitro antioxidant potentiality of crude methanol extract (CME), carbon tetrachloride fraction (CTF), petroleum ether fraction (PEF), chloroform fraction (CLF) and ethyl acetate fraction (EAF) of aerial parts of Gnaphalium luteoalbum (GL) L. Methods:The aerial parts of the GL were extracted with methanol followed by fractionation using carbon tetrachloride, petroleum ether, chloroform and ethyl acetate.The phytochemical screening of this plant was performed by using standard methods to evaluate the existence of alkaloids, carbohydrates, phenols, flavonoids, saponins, tannins, terpenoids and fixed oils.Antioxidant potentiality was estimated by, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH) and nitric oxide (NO) radical scavenging tests. Total antioxidant capacity (TAC), total phenolic content (TPC) and total flavonoid content (TFC) were also measured. Results: Phytochemical analysis of the aerial parts of GL confirmed the presence of carbohydrates, phenols, flavonoids and saponins in crude extract and its all fractions. The CME showed the highest scavenging activity (43.28%) with IC50 of 398.49 μg/mL in the DPPH radical scavenging test. The IC50 values of EAF, CME were statistically significant (P < 0.05, P < 0.01) with respect to ascorbic acid (ACA). For OH and NO radical scavenging tests maximum scavenging (48.39%, 69.64%) was also reported for CME compared to CTF, PEF, CLF and EAF. Compared to ACA, in case of OH and NO radical scavenging activities the IC50 values of CME were markedly significant (P < 0.01, P < 0.05). In the TAC test, CME showed the highest antioxidant activity (absorbance, 2.6 nm) related to other fractions. TPC was found to be the highest in the CME (115.96 mg of gallic acid equivalent/g of dried extract) rather than other fractions. The ranking order of CTF, PEF, CLF, EAF and CME for TFC was 48.67 < 55.75 < 65.29 < 71.35 < 82.29 mg quercetin equivalent/g of dried extract. Conclusion: The existing study suggested that CME of the aerial parts of GL can be used as a natural source of antioxidant which might be effective towards preventing or slowing oxidative stress related disorders.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".