INDUCIBLE NITRIC OXIDE SYNTHASE (INOS) IN MURINE PULMONARY MICROVASCULAR ENDOTHELIAL CELLS (PMEC), BUT NOT NEUTROPHILS, STIMULATES NEUTROPHIL APOPTOSIS.
Bibliographic record
Abstract
Neutrophils (PMN) migrating into the lung demonstrate delayed apoptosis. As nitric oxide (NO) regulates many aspects of PMN function, eg. adhesion, migration, we investigated the effects of iNOS on PMN caspase‐3 activity (C3A), a key enzyme in the apoptosis cascade. PMEC and bone marrow PMN were isolated from iNOS+/+ and iNOS−/− mice. PMN were cultured alone or co‐cultured with PMEC monolayers with or without LPS/IFN‐γ. At different time points, PMN or trans‐PMEC migrated PMN were collected and their C3A was measured. In unstimulated PMN, C3A increased 8.5±1.3‐fold at 4 and 8 hr, and declined at 22 hr. LPS/IFN‐γ decreased peak C3A by 40–50% vs unstimulated PMN. In trans‐PMEC migrated PMN, the increase in C3A was delayed to 22 hr. Moreover, C3A was significantly greater in PMN following migration through iNOS+/+ PMEC vs through iNOS−/− PMEC, although the migration rate was similar at all time points. Under all conditions, changes in C3A and time course were identical between iNOS−/− and iNOS+/+ PMN. These data suggests that iNOS expressed in PMEC, but not in PMN, has an important pro‐apoptotic effect on trans‐PMEC migrated PMN. Caspase‐3 activity (p mol/10 5 cells · hr) of PMN (n=4‐5) Treatment 0 hr 4 hr 8 hr 22 hr iNOS+/+ PMN
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".