The Isolation of Metabolite Compounds from Seaweed (<i>Halimeda gracillis</i>) in the Waters of Teluk Lampung as a Source of Antibacterial Compounds
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Bibliographic record
Abstract
The isolation of secondary metabolites of seaweed Halimeda gracilis was conducted. Isolation begins with 2 kg macerated dried powder Halimeda gracillis in 8 L of n-hexane solvent followed by ethyl acetate and methanol for 3 days. The solvent was filtered and followed by evaporation. Separation and purification of the isolated compounds were done by using chromatography technique. Antibacterial activity was tested by the disc diffusion method with the bacteria E. coli , S. disentriae , and B. subtilis . Isolated compounds are in the form of white crystals. Based on the spectroscopy analysis data of UV, IR, 1H-NMR, 13C-NMR, HMQC, HMBC, and COSY, the pure compounds isolated are β-sitosterol from n-hexane extract and oleic acid from ethyl acetate extract. Isolated compounds and n-hexane extracts showed non antibacterial active, while the ethyl acetate extract showed weak antibacterial active on three test bacteria at a concentration of 200,000 ppm with clear zones of 9.47; 11.09; and 8.84 mm.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.003 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it