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Record W2746510926 · doi:10.1021/acs.jmedchem.7b00463

Discovery and Optimization of 5-Amino-1,2,3-triazole-4-carboxamide Series against <i>Trypanosoma cruzi</i>

2017· article· en· W2746510926 on OpenAlexaff
Stephen Brand, Eun Jung Ko, Elisabet Viayna, Stephen Thompson, Daniel Spinks, Michael G. Thomas, Lars Sandberg, Amanda Fortes Francisco, Shiromani Jayawardhana, Victoria Smith, Chimed Jansen, Manu De Rycker, John Thomas, Lorna MacLean, Maria Osuna‐Cabello, Jennifer Riley, Paul Scullion, Laste Stojanovski, Frederick R. C. Simeons, Ola Epemolu, Yoko Shishikura, Sabrinia Crouch, Tania Bakshi, C. J. Nixon, Iain H. Reid, Alan P. Hill, Tim Underwood, Sean J. Hindley, Sharon Robinson, John M. Kelly, José M. Fiandor, Paul G. Wyatt, María Marco, Timothy J. Miles, Kevin D. Read, Ian H. Gilbert

Bibliographic record

VenueJournal of Medicinal Chemistry · 2017
Typearticle
Languageen
FieldMedicine
TopicTrypanosoma species research and implications
Canadian institutionsDiscovery Centre
FundersWellcome TrustWellcome
KeywordsBenznidazoleNifurtimoxTrypanosoma cruziChagas diseasePharmacologyVero cellTrypanocidal agentChemistryAfrican trypanosomiasisBiologyIn vitroParasite hostingVirologyTrypanosomiasisBiochemistry

Abstract

fetched live from OpenAlex

Chagas' disease, caused by the protozoan parasite Trypanosoma cruzi, is the most common cause of cardiac-related deaths in endemic regions of Latin America. There is an urgent need for new safer treatments because current standard therapeutic options, benznidazole and nifurtimox, have significant side effects and are only effective in the acute phase of the infection with limited efficacy in the chronic phase. Phenotypic high content screening against the intracellular parasite in infected VERO cells was used to identify a novel hit series of 5-amino-1,2,3-triazole-4-carboxamides (ATC). Optimization of the ATC series gave improvements in potency, aqueous solubility, and metabolic stability, which combined to give significant improvements in oral exposure. Mitigation of a potential Ames and hERG liability ultimately led to two promising compounds, one of which demonstrated significant suppression of parasite burden in a mouse model of Chagas' disease.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.315
Threshold uncertainty score0.471

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.001
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.296
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations44
Published2017
Admission routes1
Has abstractyes

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