WS1_1 Sputum Anti-neutrophil Cytoplasmic Antibodies (ANCA) in Eosinophilic Granulamatosis and Polyangiitis (eGPA)
Bibliographic record
Abstract
Objectives: Eosinophilic granulomatosis with polyangiitis (eGPA) is a systemic small-vessel vasculitis characterized by hypereosinophilia, with a pathophysiology linked to the autoinflammatory effect of anti-neutrophil cytoplasmic antiantibodies (ANCA). However, of clinically diagnosed eGPA patients only 40% are ANCA seropositive [Curr Opin Rheumatol, 2014. 26(1): p.16-23]. We hypothesized that ANCA-negative patients with severe respiratory involvement would present with ANCAs localized to the lungs. Methods: We collected matched sera and induced sputum from 20 eGPA patients (diagnosis based on 4/6 criteria by the American College of Rheumatology), 11 prednisone-dependent severe eosinophilic asthmatics, and 13 healthy volunteers. ANCA-positive patient sera were used to validate the commercial immunofluorescence (IIF) kit (Immco Diagnostics Buffalo, NY, USA) and anti-MPO ELISA. The intensity of the IIF-staining was scored by three blinded observers. Further, to negate false pANCA patterns portrayed by anti-nuclear antibodies, both ethanol, and formalin-fixed neutrophil-substrate slides, in addition to Hep-2 IIF were employed. For sputum, to reduce signal-to-noise ratio, immunoglobulins were immunoprecipitated using Protein A/G beads, and IIF was performed at 1:2 titers as per manufacturer's protocol.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".