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Record W2747741085 · doi:10.13034/jsst.v10i1.130

Le potentiel thérapeutique du CTX-1

2017· article· fr· W2747741085 on OpenAlex
Tarek Omaiche

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueJournal of Student Science and Technology · 2017
Typearticle
Languagefr
FieldImmunology and Microbiology
TopicToxin Mechanisms and Immunotoxins
Canadian institutionsnot available
Fundersnot available
KeywordsPhilosophy

Abstract

fetched live from OpenAlex

La nature est une source quasi illimite de substances possdant des actions pharmacologiques.Ces composs proviennent de plantes et de diverses espces animales.Par exemple, les serpents, les scorpions, les araignes et certains lzards et mollusques produisent des venins qui sont des mlanges complexes de molcules aux proprits biologiques varies.L'identification de ces substances et la caractrisation de leurs effets permettent de dcouvrir des molcules qui possdent souvent un potentiel thrapeutique trs significatif.Considrant cette observation, cet article est une revue de la littrature sur des travaux qui sont mens afin d'isoler de trs petites protines, appeles peptides, capables de stimuler la scrtion d'insuline.Ces peptides sont alors utiles dans le contrle du diabte.Ainsi, le venin de Naja kaouthia, un cobra de l'Asie du Sud-Est, a t le cocktail de peptides de dpart pour une tude.L'attention a t porte sur les fractions peptidiques de faibles poids molculaires et, au moyen de techniques raffines de purification, une vingtaine d'entre elles ont t isoles.Cependant, de ces fractions, une seule tait capable de stimuler la scrtion d'insuline par des cellules pancratiques et ne causait pas d'effets dltres sur les cellules.Elle est devenue la cible privilgie pour une valuation pharmacologique.Les analyses ont permis de dterminer que cette substance bioactive est la cardiotoxine-I (CTX-I), un peptide de 60 acides amins.Par la suite, les chercheurs ont vis dfinir si une portion particulire de la CTX-I est l'origine des proprits insulinotropes.Ainsi, partir de fragments synthtiss en laboratoire, ils ont dmontr qu'un segment correspondant au tiers de la molcule (acides amins 41 60 de la chane peptidique) contient les lments structuraux et chimiques ncessaires pour induire la libration d'insuline.galement, par comparaison avec d'autres toxines, la substitution d'un acide amin a t propose afin d'augmenter l'efficacit du compos.C'est ainsi qu'une nouvelle molcule synthtique, soit le fragment 41 60 de la CTX-I, portant la position 52 un acide amin modifi (lysine la place de valine), montre des proprits de stimulation de la scrtion d'insuline quivalentes celles de la molcule naturelle, tout en tant dpourvu d'effets toxiques sur des cellules-modles.In nature, substances that possess pharmacological properties are virtually limitless.These compounds originate from plants as well as different species of animals.Namely, certain snakes, scorpions, spiders, lizards and molluscs produce venoms containing a complex cocktail of varied bioactive molecules.Thus, the identification and characterization of the effects of these molecules may lead to new therapeutic avenues.This article is a revue of recent literature pertaining to the discovery of very small proteins (named peptides), capable of stimulating the secretion of insulin.Accordingly, the proteins come from the venom of the Naja kaouthia, a cobra indigenous to Southeast Asia.Using refined purification techniques, 22 low molecular weight peptide fractions were isolated and then characterized based on their ability to stimulate the secretion of insulin from pancreatic cells.Out of these peptides, a few possessed the desired attribute but only one did not show toxic actions on cells.An analysis determined that the substance was cardiotoxin-I (CTX-I), a 60-amino acid peptide.Later, using various synthetic fragments, it was uncovered that only the amino acids 41 to 60 are sufficient to stimulate the insulin secretion.Finally, by comparing CTX-I with other venom toxins, researchers proposed the substitution of one amino acid in order to increase the effectiveness of the compound.The newly formed synthetic peptide, containing the 41 to 60 amino acid segment of CTX-I and a residue substitution at position 52 (lysine instead of valine), exhibited potent insulinotropic properties while maintaining cell viability.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesScience and technology studies
Consensus categoriesScience and technology studies
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.483
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.000
Science and technology studies0.0030.007
Scholarly communication0.0000.001
Open science0.0020.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.270
Teacher spread0.260 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it