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Record W2748894775 · doi:10.1038/s41598-017-10147-x

The Parkinson’s disease-associated GPR37 receptor interacts with striatal adenosine A2A receptor controlling its cell surface expression and function in vivo

2017· article· en· W2748894775 on OpenAlexaff
Xavier Morató, Rafael Luján, Marc López‐Cano, Jorge Gandía, Igor Štagljar, Masahiko Watanabe, Rodrigo A. Cunha, Víctor Fernández‐Dueñas, Francisco Ciruela

Bibliographic record

VenueScientific Reports · 2017
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAdenosine and Purinergic Signaling
Canadian institutionsUniversity of Toronto
FundersFonds Wetenschappelijk OnderzoekInstituto de Salud Carlos IIIJunta de Comunidades de Castilla-La ManchaEuropean CommissionUniversitat de BarcelonaFundació la Marató de TV3
KeywordsAgonistAdenosine A2A receptorReceptorCell biologyStriatumChemistryInverse agonistOrphan receptorG protein-coupled receptorPharmacologyAdenosine receptorNeuroscienceBiologyDopamineBiochemistryTranscription factor

Abstract

fetched live from OpenAlex

Abstract G protein-coupled receptor 37 (GPR37) is an orphan receptor associated to Parkinson’s disease (PD) neuropathology. Here, we identified GPR37 as an inhibitor of adenosine A 2A receptor (A 2A R) cell surface expression and function in vivo . In addition, we showed that GPR37 and A 2A R do oligomerize in the striatum. Thus, a close proximity of GPR37 and A 2A R at the postsynaptic level of striatal synapses was observed by double-labelling post-embedding immunogold detection. Indeed, the direct receptor-receptor interaction was further substantiated by proximity ligation in situ assay. Interestingly, GPR37 deletion promoted striatal A 2A R cell surface expression that correlated well with an increased A 2A R agonist-mediated cAMP accumulation, both in primary striatal neurons and nerve terminals. Furthermore, GPR37−/− mice showed enhanced A 2A R agonist-induced catalepsy and an increased response to A 2A R antagonist-mediated locomotor activity. Overall, these results revealed a key role for GPR37 controlling A 2A R biology in the striatum, which may be relevant for PD management.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.110
Threshold uncertainty score0.690

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.226
Teacher spread0.217 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations45
Published2017
Admission routes1
Has abstractyes

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