Genetic Assessment of <i>Amaranthus</i> Linn. Genotypes in Treatment Combinations of <i>Glomus clarum</i> and <i>Leucaena leucocephala</i> Lam. Using Simple Sequence Repeat (SSR) Marker
Bibliographic record
Abstract
The molecular and genetic assessments of the seeds of five Amaranthus genotypes in four treatment combinations of Arbuscular mycorrhiza Fungus ( Glomus clarum ) and Green manure ( Leucaena leucocephala ) were evaluated using SSR maker with four primers. The experiment was laid out in a complete randomized design with four replicates. Heritability of growth traits were higher than yield traits for treated Amaranthus genotypes. The plant height had strong positive correlation with stem length (r=0.8919), inflorescent length (r=0.6150) and inflorescent width (r=0.6004). Prin 1 accounted for the highest variation with proportion of 0.4197 and eigenvalue of 5.8765. The study detected the polymorphism, gene and allelic diversity and established phylogenetic relationships among the Amaranthus population. NGBO1271 treated with G. clarum + L . leucocephala had the highest concentration of extracted DNA (4039.2ul), while NGBO1234 treated with G. clarum and NGBO1234 treated with L . leucocephala had highest genomic DNA nanodrop of 2.14 gl. A total of 15 alleles were detected and number of allele per primer ranged from 3 to 5 with mean value of 3.75. The highest allelic frequency of 0.75 was recorded for ASAAC005 and ASAAC006. However, ASAAC001 primer was polymorphic (66.86%), with highest allele number and allele diversity of 5.0000 and 0.7200 respectively. The result from Nei’s coefficient also confirmed genetic variation based on the total gene diversity and gene diversity per locus. The dendogram among the treated Amaranthus genotypes showed their phylogenetic relationship which can be useful in selection of closely related genotypes for desirable traits. Therefore, NGBO1234 and NGBO1271, G. clarum and L . leucocephala could be suitable genotypes and further explored in genetic improvement of crops. Also, ASAAC001 primer could be considered in molecular breeding of Amaranthus and other vegetables.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".