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<scp>LHON</scp>: A look into nuclear and environmental factors; What is “sufficient”?

2017· article· en· W2751719375 on OpenAlexaff
A. Sadun, Fred N. Ross‐Cisneros, Jack J. Tian, K Anderson, A. Ray Irvine, Rustum Karanjia, Chiara La Morgia, Meagan J. McManus, David Wallace, Valério Carelli

Bibliographic record

VenueActa Ophthalmologica · 2017
Typearticle
Languageen
FieldEngineering
TopicNuclear and radioactivity studies
Canadian institutionsOttawa HospitalUniversity of Ottawa
Fundersnot available
KeywordsPsychologyMedicineOptometry

Abstract

fetched live from OpenAlex

Purpose In Leber's Hereditary Optic Neuropathy ( LHON ) mutations of the mt DNA , are necessary but not sufficient for visual loss. We studied nuclear/environmental interactions in patients and in the Wallace LHON mouse model, to consider what is “sufficient”. The LHON mutation causes a modest decrease in ATP production, but a large increase in ROS production. Nuclear genetic and environmental factors may play upon these factors Methods We noted in the data of Kirkman et al (Brain 2009) that heavy smoking mitigated visual loss by delaying disease onset. We reproduced these peculiar results (Carelli et al, Brain 2013). We also studied the ND 6 mutant Wallace mouse model of LHON . 30 mice of 12 groups were sacrificed at 23 months including ND 6 mutant vs ND 6 wildtype and nicotinamide nucleotide transhydrogenase‐ NNT +/+ vs ‐/‐ ( NNT deletion), under different environments of air and smoke. 10 experiments compared the axonal nerve fiber spectrums. Results In our patient pedigrees, heavy smoking also mitigated visual loss, but we saw in this, the existence of two subtypes of LHON . Type I, with an abrupt and severe loss of vision in the teens, was characterized by severe losses of both structure and function. Type II occured decades later with a more insidious onset, with less structural than functional loss. Type II patients were more likely to smoke. In the mice, we found the predominant loss of small fibers in NNT −/− mice carrying the ND 6 mutation which were exposed to smoke. Conclusions Both nuclear modifiers and environmental factors in combination with LHON mutations can lead to vision loss. NNT is a transhydrogenase that couples hydride transfer from NAD (H) to NADP (+) and proton translocation across the inner mitochondrial membrane. NNT is potentially an important nuclear modifying gene for LHON .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.400
Threshold uncertainty score0.846

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0000.001
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.228
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2017
Admission routes1
Has abstractyes

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