Soil and Phyllosphere Microorganisms of the Rapeseed (Brassica napus L.) Holobiont Involved in Chloromethane Emissions
Bibliographic record
Abstract
Brassica napus, or rapeseed, is one major oilseed crop in Europe and Germany and is used for food, feed, and Biodiesel production. Chloromethane (CH3Cl) is the most abundant halogenated organic compound in the atmosphere and triggers the chlorine-mediated destruction of the ozone layer. Anthropogenic sources became negligible because of taken measures according to the Montreal Protocol (1987) and therefore natural sources such as plants became more relevant for the global chloromethane budget. The actual global budget is imbalanced through missing sinks, which may be activities of soil and phyllosphere microbiomes which together with the plant is considered as the holobiont. The amount of CH3Cl from crops has not been addressed and might even increase under stresses such as elevated soil salinization and temperature. We proved that B. napus (rapeseed) plants emit CH3Cl. Certain methylotrophs (microorganisms that utilize one-carbon compounds) degrade CH3Cl and can gain a selective advantage while colonizing plants. Moreover, methylotrophs thrive in the rhizosphere of grassland plants. The rapeseed associated microbiome likely harbours methylotrophs that degrade CH3Cl. Therefore, we addressed in this study the rapeseed holobiont to resolve its response to stressors such as salt and temperature in regard to CH3Cl emission. We addressed in our project the following objectives: (i) To measure CH3Cl emission rates from single B. napus holobionts under different NaCl and temperature stress levels in pot experiments, (ii) to assess the B. napus microbiome and its CH3Cl degradation ability through amplicon sequencing of bacterial 16S rRNA genes and functional gene markers. The summer cultivar MAKRO was used as a model organism. We identified methylotrophs that responded to salt and temperature stress conditions in the phyllo- and rhizosphere of rapeseed and correlated those to the observed net emission rates.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".