Noncovalent Immobilization of Optimized Bacterial Cytochrome P450 BM3 on Functionalized Magnetic Nanoparticles
Bibliographic record
Abstract
A stable insoluble P450 BM3 system that can be easily separated from the reaction medium for recycling, while using less-expensive cofactors (NADH and BNAH) than costly NADPH, can represent a promising biocatalyst in industrial applications. In this context, the present work investigates the immobilization of double mutant cytochrome P450 BM3 (R966D/W1046S) by adsorption and cross-linking-adsorption on Ni 2+ -functionalized magnetic nanoparticles (MNPs). By oxidizing NADH or BNAH, the immobilized BM3 succeeded in hydroxylating the substrates (10- p NCA and myristic acid) to a similar degree as the free enzyme. The adsorbed enzyme showed 88% hydroxylation residual activity after five reaction cycles (five continuous days), which was increased to 100% by cross-linking the adsorbed enzyme. In addition, the cross-linked-adsorbed enzyme kept 41% of its initial activity toward NADH after one month of storage at 4 °C, while the free enzyme showed only 31% residual activity after 1 week and was inactive afterward. The results of this work highlighted that the appropriate choice of the enzyme-support-cofactor system can result in an active, stable, and recyclable biocatalyst, which could attract growing industry interest.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".