Enlargement of the <scp>WHO</scp> international repository for platelet transfusion‐relevant bacteria reference strains
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Background and Objectives Interventions to prevent and detect bacterial contamination of platelet concentrates ( PC s) have reduced, but not eliminated the sepsis risk. Standardized bacterial strains are needed to validate detection and pathogen reduction technologies in PC s. Following the establishment of the First International Reference Repository of Platelet Transfusion‐Relevant Bacterial Reference Strains (the ‘repository’), the World Health Organization ( WHO ) Expert Committee on Biological Standardisation ( ECBS ) endorsed further repository expansion. Materials and Methods Sixteen bacterial strains, including the four repository strains, were distributed from the Paul‐Ehrlich‐Institut ( PEI ) to 14 laboratories in 10 countries for enumeration, identification and growth measurement on days 2, 4 and 7 after low spiking levels [10–25 colony‐forming units ( CFU )/ PC bag]. Spore‐forming ( Bacillus cereus PEI ‐B‐P‐07‐S, Bacillus thuringiensis PEI ‐B‐P‐57‐S), Gram‐negative ( Enterobacter cloacae PEI ‐B‐P‐43, Morganella morganii PEI ‐B‐P‐74, PEI ‐B‐P‐91, Proteus mirabilis PEI ‐B‐P‐55, Pseudomonas fluorescens PEI ‐B‐P‐77, Salmonella choleraesuis PEI ‐B‐P‐78, Serratia marcescens PEI ‐B‐P‐56) and Gram‐positive ( Staphylococcus aureus PEI ‐B‐P‐63, Streptococcus dysgalactiae PEI ‐B‐P‐71, Streptococcus bovis PEI ‐B‐P‐61) strains were evaluated. Results Bacterial viability was conserved after transport to the participating laboratories with one exception ( M. morganii PEI ‐B‐P‐74). All other strains showed moderate‐to‐excellent growth. Bacillus cereus , B. thuringiensis , E. coli , K. pneumoniae , P. fluorescens , S. marcescens , S. aureu s and S. dysgalactiae grew to >10 6 CFU /ml by day 2. Enterobacter cloacae , P. mirabilis , S. epidermidis , S. bovis and S. pyogenes achieved >10 6 CFU /ml at day 4. Growth of S. choleraesuis was lower and highly variable. Conclusion The WHO ECBS approved all bacterial strains (except M. morganii PEI ‐B‐P‐74 and S. choleraesuis PEI ‐B‐P‐78 ) for repository enlargement. The strains were stable, suitable for spiking with low CFU numbers, and proliferation was independent of the PC donor.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it