Isolation and ex vivo expansion of murine bone marrow-derived group 2 innate lymphoid cells (MUC8P.734)
Bibliographic record
Abstract
Abstract Group 2 innate lymphoid cells (ILC2) are recently discovered lymphocyte subpopulations that have innate ability to produce large quantities of a specific set of cytokines regulating innate and adaptive immune responses. Importantly, deregulated ILC2 responses are implicated in detrimental type 2 immunopathologies such as allergies, asthma and atopic dermatitis, as well as fibrotic disease, both in rodents and humans. However, the progress in our understanding of ILC2 biology has been hampered by their scarcity at steady state as well as infected and inflamed tissues. Here, a simple, efficient and reliable method is presented to isolate murine ILC2 from the bone marrow by flow cytometric cell sorting and further cultivate them using a defined cytokine cocktail, yielding a 500-fold expansion. The resulting ILC2 constitute a pure cell population and show the same phenotypic and functional characteristics as ILC2 in vivo. Thus, this protocol enables a high yield of ILC2 from murine bone marrow and thereby provides sufficient ILC2 numbers for standard cell and molecular biology assays. An in-depth understanding of the cellular and molecular mechanisms of ILC2 is essential for the development of new strategies and drugs to fight unwanted type 2 immunopathologies and fibrotic disease.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".